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为了进一步认识花生种间杂交异源多倍体进化过程中的基因表达变化规律,采用cDNA-HFO-TAG技术,研究花生种间杂交组合(四倍体栽培种‘仲恺花4号’×二倍体野生种Arachis. diogio)杂种F_1和早期多倍体世代S0~S3的基因表达变化情况。14条HFO-TAG引物共扩增出121条cDNA片段,其中差异片段84个,主要包括三种类型:亲本转录物完全沉默(3个),双亲转录物在后代部分材料中沉默(59个)和新转录物激活(22个),上述变化在F1代即开始发生。筛选其中大小为500~2 000 bp的35个TDFs进行克隆测序,有27个和NCBI数据库中已录入的基因具有较高的相似性,包括抗逆相关基因(10条)、未知功能蛋白基因(8条)、能量与代谢相关基因(7条)和转录因子相关的基因(2条)。这些研究结果进一步表明在花生种间杂交异源多倍化早期发生着快速、剧烈的基因表达变化,从中获得的差异基因片段,有助于了解花生属种间杂交异源多倍化早期分子机制变化,这对有效利用野生花生种质优异基因具有重要意义。cDNA-HFO-TAG技术简单、有效且实用,完全适用于花生属基因表达变化研究,可以作为花生属及其它物种基因表达变化分析技术的有效补充。 相似文献
64.
以蒙古黄芪-根腐病菌(Fusarium solani和F. acuminatum)为互作体系,测定苯丙烷途径关键酶苯丙氨酸解氨酶(PAL)、肉桂酸-4-羟化酶(C4H)及4-香豆酸-辅酶A连接酶(4CL)活性的变化,以期获得黄芪抗根腐病菌侵染机制的信息,为抗病育种工作提供参考。试验采用幼苗浸根法接种病菌,分光光度法测定3种酶的动态变化。结果表明,F. solani侵染黄芪后,PAL和C4H活性与对照相比明显升高,并在7、21天出现2个酶活高峰;4CL活性则随着时间的延长持续上升,7、21天时也显著高于对照。F. acuminatum侵染黄芪后,PAL、C4H和4CL的活性均在21天显著高于对照。可见2种病原菌在侵染黄芪过程中,苯丙烷途径的关键酶被不同程度地激活,但2种病菌激活各酶的时间和规律有所差异,表明黄芪对这2种病菌的抗性机制明显不同。 相似文献
65.
高分子量谷蛋白亚基(high-molecular-weight glutenin subunits,HMW-GS)是小麦籽粒贮藏蛋白的重要组成成分,其数量和质量与品质密切相关。聚合优质HMW-GS可改良强筋小麦制品面包品质,相反HMW-GS缺失可能在弱筋小麦制品饼干、糕点或其他特色食品品质改良上具有重要应用价值。本文从小麦HMW-GS缺失的类型和机制、贮藏蛋白组分和蛋白体发育、面粉和面团品质效应以及食品加工品质改良等方面进行了综述,分析了当前小麦HMW-GS缺失研究利用中存在的问题,并对未来研究方向进行了展望。 相似文献
66.
以湘烟5号为材料,通过盆栽试验,探讨不同浓度植物提取物对烤烟主要化学成分及致香物质含量的影响。结果表明,喷施栀子花和玉兰花汁液提取物有利于烟叶原料主要化学成分含量的改善,与对照相比,喷施处理的上部橘黄二级(B2F)和中部橘黄三级(C3F)烟叶还原糖含量分别高15.0%~21.1%和14.3%~17.7%,钾含量分别高5.7%~13.9%和7.4%~14.0%;与对照相比,喷施处理的烟叶的石油醚提取物、类胡萝卜素降解物、降解产物、茄酮、非酶棕色化反应产物、芳香族氨基酸裂解产物、新植二烯和中性致香物质总量,上部橘黄二级(B2F)烟叶分别高10.0%~20.9%、11.2%~20.9%、9.6%~20.2%、9.8%~21.1%、10.8%~22.5%、11.2%~23.0%、14.9%~24.7%和11.9%~22.1%,中部橘黄三级(C3F)烟叶分别高13.5%~24.1%、11.3%~21.0%、11.4%~20.0%、10.8%~18.7%、13.1%~21.2%、10.1%~19.1%、17.5%~27.0%和18.1%~27.6%;烟叶感官质量评价权重得分高于对照。栀子花和玉兰花汁液提取物对烟叶主要化学成分及致香物质含量的作用效果以施用量3.5~5.0g/株的处理最佳。 相似文献
67.
We analysed the metabolic rate of large individuals (LIs) and small individuals (SIs) (based on body weight) of 80‐day‐old specimens of Portunus trituberculatus injected with 40 mg/kg florfenicol (FLR). The results showed that FLR contents in the gills and hepatopancreas were higher in the SIs than in the LIs. The activities of erythromycin N‐demethylase and 7‐ethoxyresorufin‐O‐deethylase related to cytochrome oxidase P450 (CYP) were induced after FLR injection and showed a size‐dependent pattern; however, no significant difference in aminopyrine N‐demethylase activity was found between the LIs and SIs. The activity of the phase II enzyme glutathione S‐transferase (GST) increased in the hepatopancreas after FLR injection, and it was higher in the SIs than the LIs. Moreover, induction of CYP2, CYP3, GST, ABCB and ABCG was observed in the first 24 hr after FLR injection. FLR caused more biomolecular damage in the SIs than the LIs, displaying an obvious body weight‐ and time‐dependent pattern. In summary, the SIs exhibited faster FLR uptake and slower depuration in this species, and FLR caused more biomolecular damage to the SIs. 相似文献
68.
Xu Gao Han Gil Choi Seo Kyoung Park Ju Hyoung Kim Ok Hwan Yu Ki Wan Nam 《Aquaculture Research》2019,50(3):856-861
Juvenile sporophytes and gametophytes of Ecklonia stolonifera were incubated in combinations of three pCO2 levels (360, 720 and 980 ppmv) and two temperatures (10 and 15°C for sporophytes; 15 and 20°C for gametophytes) to examine potential effects of climate change on photosynthesis and growth. Sporophytes had significantly higher maximum quantum yields (Fv/Fm) and maximum relative electron transport rates (rETRmax) at 720 ppmv than 360 and 980 ppmv. Also, these parameters were significantly lower at higher temperature of 15°C than at 10°C. Growth of female gametophytes was maximal at 360 ppmv rather than enriched pCO2 levels. Female gametophytes had significantly lower growth at higher temperature of 20°C than at 15°C. These results indicate effects of elevated pCO2 varied between generations: stimulating sporophytic photosynthesis and inhibiting gametophytic growth. Ocean acidification and warming would constitute a grave threat to seedling cultivation of E. stolonifera caused by growth inhibition of gametophytes at high pCO2 levels and temperatures. 相似文献
69.
以贵州省不同年代酿酒用糯高粱品种(系)为研究对象,采用随机区组试验设计,对其农艺性状、产量构成以及抗性差异进行分析,结果表明:随着年代更替,贵州省酿酒用糯高粱单产逐步提高。新品种(红缨子、黔高8号)较老品系(黑壳糯、红壳糯)平均单产增幅25%以上;穗粒数、穗粒重增加显著,增幅分别为51.7%、62.7%;主穗一、二级枝梗数增加幅度分别为19.8%、62.7%;株高降低24.9%,茎粗增加9.5%;节间数增加0.9个,节间长缩短13.3cm;单株叶面积和叶面积指数增加显著;倒伏率、发病率下降显著,成穗率明显提升。各指标相关性分析显示,产量与穗粒数、穗粒重,穗粒数与主穗一、二级枝梗数呈极显著正相关;产量与株高、倒伏率极显著负相关,与茎粗正相关,相关系数分别为-0.981、-0.970和0.928,株高、茎粗与倒伏率相关显著,相关系数分别为0.964和-0.910;产量与叶面积指数正相关,与发病率显著负相关。总的结果表明,主穗一、二级枝梗数的增加提高了糯高粱的穗粒数,植株株高的降低、茎粗的增加促进了抗倒性的提升,抗病性的提升保证了后期叶片光合作用的持续进行,最终提高了糯高粱产量。 相似文献
70.
Xiao‐Qing Dong Gui‐Juan Qu Yu‐Ke Chen Gui‐Qin Wang Dan Jiang 《Aquaculture Research》2019,50(7):1752-1761
A 60‐day feeding trial was conducted to examine the effects of different levels (0, 100, 200, 400 and 600 mg/kg) of antimicrobial peptides on growth, protease activity of foregut, the morphology of foregut villi and related genes mRNA expression level in the common carp (Cyprinus carpio). The results showed that the feed of antimicrobial peptides promote common carp growth, and the optimal dosage of antimicrobial peptides is 200–333 mg/kg in the common carp feed. The protease activity of 200 and 400 mg/kg groups were significantly higher than the control and other groups (p < 0.05). The foregut villus height with 100, 200 and 400 mg/kg antimicrobial peptide groups were significantly higher than control group (p < 0.05). The crypt depth of 200 and 400 mg/kg antimicrobial peptide groups were significantly lower than control group (p < 0.05). The ratio of villus height and crypt depth of 100, 200 and 400 mg/kg antimicrobial peptide groups were significantly higher than control group (p < 0.05). The ratio with 600 mg/kg group was significantly lower than the control group (p < 0.05). The IGF‐I gene expression level of 200 mg/kg and 400 mg/kg groups were significantly higher than the control group and 600 mg/kg group (p < 0.05). The IL‐1β gene expression level of 100 mg/kg and 200 mg/kg groups were significantly higher than the control group (p < 0.05). These results indicated up‐regulation of growth and immune related genes in antimicrobial peptides fed common carp. Correlation analysis showed that IGF‐I mRNA and IL‐1β mRNA were positively correlated with SGR. IL‐1β mRNA and FCR were significantly negative correlated. It indicated that growth and immune gene common regulated the growth of the carp under antimicrobial peptides intervention. In conclusion, antimicrobial peptides can improve growth and related genes mRNA expression in the common carp. Further studies using molecular biological technique or immunologic methods are required to conclude that antimicrobial peptides are beneficial in common carp. 相似文献